Search results for "blood–testis barrier"
showing 3 items of 3 documents
Nandrolone decanoate interferes on testosterone biosynthesis and alters blood-testis barrier.
2018
Nandrolone decanoate (ND) is a synthetic testosterone analogue considered one of the most commonly abused anabolic androgenic steroids by adolescents and athletes. ND is alleged to promote an increase in muscle mass and improves both physical appearance and sporting performance, but ND abuse is often associated with serious adverse effects, interfering with the endocrine system and the reproductive system. In a previous study, we demonstrated that ND treatment of Leydig cells interferes with the biosynthesis of testosterone in a dose increase-dependent fashion (1). As a consequence of the results obtained in vitro, in this study an animal model was utilized to better understand the side eff…
Nandrolone decanoate interferes with testosterone biosynthesis altering blood-testis barrier components
2017
Abstract The aim of this study was to investigate whether nandrolone decanoate (ND) use affects testosterone production and testicular morphology in a model of trained and sedentary mice. A group of mice underwent endurance training while another set led a sedentary lifestyle and were freely mobile within cages. All experimental groups were treated with either ND or peanut oil at different doses for 6 weeks. Testosterone serum levels were measured via liquid chromatography–mass spectrometry. Western blot analysis and quantitative real‐time PCR were utilized to determine gene and protein expression levels of the primary enzymes implicated in testosterone biosynthesis and gene expression leve…
Ultrastructural changes of the intercellular relationship in impaired human spermatogenesis
1980
In seven hypo- or aspermic patients, electron microscopic investigations of the intercellular connections of the seminiferous tubule were performed. The analysis of cell junctions of Sertoli cells and germ cells revealed irregularities of the Sertoli-cell junctions, hypoplasias of occluding junctions, hypo- and hyperplasias of the Sertoli-spermatid cell junctions and abnormal formation of Sertoli cell junctions with early spermatids, spermatocytes, and spermatogonia. Gap junction-like cell membrane specializations were very rare. Intercellular cytoplasmic bridges of germ cells were always present together with these cells. One hypoplastic bridge connecting two spermatogonia was found. The r…